Disorders of FZ-CRD; insights towards FZ-CRD folding and therapeutic landscape

CKIɛ/discs overgrown Promotes Both Wnt-Fz/β-Catenin and Fz/PCP Signaling in Drosophila

The related Wnt-Frizzled(Fz)/b-catenin and Fz/planar cell polarity (PCP) pathways are essential for the regulation of numerous developmental processes and are deregulated in many human diseases. Both pathways require members of the Dishevelled (Dsh or Dvl) family of cytoplasmic factors for signal transduction downstream of the Fz receptors. Dsh family members have been studied extensively, but ...

Clinical utility of thresholds versus CRD

Since food challenge is time consuming and not always without a risk for the patient, surrogate parameters have been introduced. Among the best studied are case history, size of Skin Prick Test and the level of specific IgE towards a food allergen. In the later case, decision points (ie. the level of specific IgE above which there would be a 95 % probability of the patient being challenge posit...

The role of the cysteine-rich domain of Frizzled in Wingless-Armadillo signaling.

The Frizzled (Fz) receptors contain seven transmembrane helices and an amino-terminal cysteine-rich domain (CRD) that is sufficient and necessary for binding of the ligands, the Wnts. Recent genetic experiments have suggested, however, that the CRD is dispensable for signaling. We engineered fz CRD mutant transgenes and tested them for Wg signaling activity. None of the mutants was functional i...

Regulation of RNA-binding by KH domains of CRD-BP

The ability of its four heterogeneous nuclear ribonucleoprotein-K-homology (KH) domains to physically associate with oncogenic mRNAs is a major criterion for the function of Coding Region Determinant-Binding Protein (CRD-BP). However, the particular RNA binding role of each of the KH domains remains largely unresolved. Here, we mutated the first glycine to an aspartate in the universally conser...

CRD: A New Data Mining Method in Deductive Databases